extraction and identification of phytoestrogen compounds, ellagic and syringic acids from pomegranate peel

in this work, fresh pomegranate peel of three dominant iranian cultivaris was extracted by soxhlet extraction method via different four solvents. the extracts were analyzed by high performance liquid chromatography (hplc), using ultraviolet-visible spectrometric detection. in this study, the extraction yields and presence of seven phytoestrogenic compounds, two tannic acids and their quantities in extracts were evaluated. the compounds were identified and quantified according to their retention times with external standards. comparison of fresh pomegranate peel of three cultivars indicated that yield of extraction from poostgoliyemalasesaveh variety was more than other two cultivars and the yield of extraction of extracts obtained from the mix solvent was more than three other solvents (p≤0.05). hplc analysis indicated besides ellagic and syringic acids, some of phytoestrogens such as esteriol (up to 7.45 mg/100 g fresh peel), testosteron and α-estradiol (up to 8.43 mg/100 g fresh peel) were also found in extract of pomegranate peels. the results show that, between two tannic acids, high value of ellagic acid (up to 103/0 mg/100g fresh peel of post siyahe-ardestan) were recognized in all extracts.